AbstractAfter feeding Atlantic salmon,Salmo salarL., five graded amounts of wheat starch from 0 to 310 g kg−1, low but increased levels of glycogen in kidney, heart and gills were determined. No variations were found in proximate or glycogen compositions of muscle, whereas the liver composition reflected the diet composition. Whole‐body homogenates varied in dry matter and lipid levels; the variation was according to g lipid eaten per fish. Plasma glucose levels ranged above average levels only when the starch level in the feed was higher than 220 g kg−1. Although feed intake and thereby lipid intake increased as dietary carbohydrate increased in the present experiment, no differences in plasma concentrations of cholesterol and only small changes in triglycerides were determined. The increase in dietary carbohydrate was balanced with protein, and total plasma protein concentrations followed the decrease in feed protein content. Haematocrit, haemoglobin, mean cell volume and mean cell haemoglobin all showed significantly reduced levels as dietary starch increased, indicating a fibre effect from ‘left‐over’ starch in the intestine followed by reduced absorption of divalent ions, s