Histological Observations of Dental Tissues Using the Confocal Laser Scanning Microscope
作者:
KabasawaMiyako,
EjiriSadakazu,
HanadaKooji,
OzawaHidehiro,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1995)
卷期:
Volume 70,
issue 2
页码: 66-69
ISSN:1052-0295
年代: 1995
DOI:10.3109/10520299509108319
出版商: Taylor&Francis
关键词: Villanueva bone stain;ground section;confocal laser scanning microscopy
数据来源: Taylor
摘要:
To investigate the time course of mineralization in undecalcified dental tissues, calcein-and tetracycline-labeled rat maxillary molar sections were stained with Villanueva bone stain en bloc, embedded in methyl-methacrylate (MMA), ground to 50μm thickness, and observed by confocal laser scanning microscopy (CLSM). This method allowed observation of dental structures including odontoblasts, pulp cells and periodontal ligament, and dentinal tubules and enamel rods at high resolution; labeled enamel, dentine, and cementum could be observed simultaneously regardless of section thickness. CLSM permitted simultaneous observation of both the components of calcified tissue and the cellular components of dental tissues, and assessment of the mineralization time course of hard tissues labeled by tetracycline or calcein. The technique is useful for both assessing the elements composing dental structure and observing the histological dynamics by which dental structure develops.
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