Purpose.To evaluate the efficacy of dextran in balanced salt solution (BSS) as a preparation of eye bank corneas for experimental surgeries.Methods.We used 12 eye bank eyes that were unsuitable for transplant. The corneas were removed from the globe and affixed to a Hanna artificial anterior chamber. Four concentrations of dextran–BSS (10%, 15%, 20%, and 30% [± 0.56%]) were used to dehydrate the corneas, and corneal thickness was assessed with an ultrasonic pachymeter (±5 &mgr;m) at varying time-intervals over 3 hours.Results.The corneas were thinned to an average thickness of 477 ± 54 &mgr;m, 430 ± 54 &mgr;m, 406 ± 61 &mgr;m, and 391 ± 52 &mgr;m at average times of 75 ± 0 minutes, 85 ± 23 minutes, 60 ± 12 minutes, and 45 ± 0 minutes for the 10%, 15%, 20%, and 30% concentrations, respectively. Corneal thickness was stabilized in both a 15% and a 20% solution. The data was fit to exponential curves until a minimum value was reached, after which linear regression analysis was used to determine the slopes of the data. The slopes for the 15% and 20% concentrations were not significantly different from 0 (p= 0.5 andp= 0.4, respectively). The slopes for each set of data from the 10% and 30% concentrations were significantly different from each other (p= 0.010 andp= 0.001, respectively).Conclusions.A solution of 20% dextran in BSS is effective for dehydrating eye bank corneas to a quasi-normal physiologic thickness and for maintaining the thickness for a sufficient amount of time. Both the posterior and anterior sides of the cornea should be exposed to the solution for 60 minutes.