The relevance of various tests for the study of specificity in immunocytochemical staining: A review
作者:
P. J. Van Der Sluis,
G. J. Boer,
期刊:
Cell Biochemistry and Function
(WILEY Available online 1986)
卷期:
Volume 4,
issue 1
页码: 1-17
ISSN:0263-6484
年代: 1986
DOI:10.1002/cbf.290040102
出版商: John Wiley&Sons, Ltd.
关键词: Immunocytochemistry;serum specificity tests
数据来源: WILEY
摘要:
AbstractFactors determining the specificity of immunocytochemical (ICC) tissue stainings as well as the various tests to study these factors are discussed. Since every specificity test only deals with particular aspects of the ICC procedure, a practical sequence of known test methods is proposed, which enables the determination of the specificity of the ICC tissue staining and, after possibly needed antiserum purification steps, may result in a monospecific staining. It is made clear that such a sequence has always to include a tissue‐spectrum affinity test, in which the spectrum of tissue antigens is controlled for antibody binding. A variety of such tests, consisting of separation of tissue compounds, fixation, and ICC detection, are discussed as well as their pros and cons with respect to their predictability for the actual serum specificity in the tissue sectio
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