The concentration of glucose in aqueous solutions was monitored, using a blood glucose meter, as small weighed cyclodextrin portions were added. Equilibrium constants for the formation of inclusion complexes were calculated. A binding constant determined by this method has been verified by a technique involving competition of the guest with methyl orange indicator dye. ΔH0values for the reactions were estimated using calorimetry. These values along with ΔG0provided estimates of the standard entropies. The values calculated compare favorably with those from a previously determined empirical equation. The possibility of hydrogen bond formation is suggested by the large negative enthalpies as well as by molecular mechanics calculations.Keywords: α-, β-cyclodextrin, glucose, binding constants.