Genome size inHedera helixL.- a clarification
作者:
Renate Obermayer,
Johann Greilhuber,
期刊:
Caryologia
(Taylor Available online 2000)
卷期:
Volume 53,
issue 1
页码: 1-4
ISSN:0008-7114
年代: 2000
DOI:10.1080/00087114.2000.10589175
出版商: Taylor & Francis Group
关键词: Feulgen densitometry;flow cytometry;genome size;Hedera;H. canariensis;H.colchica;H. helix
数据来源: Taylor
摘要:
The correct genome size inHedera helixL. has long been problematic, because several authors have reported nuclear DNA content plasticity in connection with phase change from juvenile to adult. Although this has been disproved, nevertheless uncertainty existed on the correct genomic DNA amount, due to one significantly higher flow-cytometric estimate in the more recent literature. The present analysis is concerned with this problem of a possibly variable genome size within the species or other reasons for this discrepancy. Plants of typicalH. helix (2n = 48),H. helixf.arborescens (2n = 48),H. canariensisWilld.(2n = 48) andH.colchica (2n = 192) were measured with flow cytometry (propidium iodide staining) and video based Feulgen densitometry for genome size. Internal standards used wereGlycine max(flow cytometry, 2C taken as 2.226 pg) and Pisum sativum (Feulgen densitometry, 2C taken as 8.84 pg). With flow cytometry, theH. helixvarieties andH. canar-iensiswere statistically invariant,1.263-fold of G.maxwith a 2C value of 2.81 pg. Hedera colchica was 4.895—fold of G. max with a 2C value of 10.90 pg in agreement with its higher poly-ploidy level. Feulgen estimates were slightly higher but basically in agreement with these data. Neither value is close to the deviating value for H.helixin the literature mentioned above, that must remain unsupported.
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