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Recording Integrating Photoelectric and Radioactive Scanner for Paper Electrophoresis and Chromatography

 

作者: E. L. Durrum,   S. R. Gilford,  

 

期刊: Review of Scientific Instruments  (AIP Available online 1955)
卷期: Volume 26, issue 1  

页码: 51-56

 

ISSN:0034-6748

 

年代: 1955

 

DOI:10.1063/1.1771240

 

出版商: AIP

 

数据来源: AIP

 

摘要:

A versatile automatic scanner for paper strips which records directly optical density or radioactivity is built around a servo type recorder (Brown). Interference filters provide a narrow band light source. For scanning in the visible or ultraviolet where monochromatic light is desired the monochromator of a Beckman model DU spectrophotometer replaces the filters. The instrument incorporates positive synchronization of strip and recording paper, sequential scanning of strips to provide an optical density curve followed by superposition of the integral of this curve. Areas are easily calculated without the need for planimetry. The slits of varying size are interchangeable and are designed for either optical or radioactive scanning. For medium levels of activity a Geiger‐Mu¨ller tube is interchangeable with the photocell, while for low activities, optical scanning of autoradiographs may be performed.

 

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