Distribution of Radioactivity in Tissues after the Intravenous Injection of Free and Acrylic Particle Bound-Porcine125I-Neurophysin-I into Rats and Rabbits
作者:
W.B. Watkins,
S. Reddy,
H.K. Ellis,
期刊:
Neuroendocrinology
(Karger Available online 1975)
卷期:
Volume 18,
issue 3
页码: 217-232
ISSN:0028-3835
年代: 1975
DOI:10.1159/000122401
出版商: S. Karger AG
关键词: Radioiodinated porcine neurophysin-I;Rats and rabbits;Tissue distribution of radioactivity;Acrylic particles;Half-life;Immunohistology
数据来源: Karger
摘要:
Porcine neurophysin-I iodinated with Na125I was injected intravenously into rats and rabbits, and the rate of disappearance of radioactivity from the peripheral system was measured. Radioactively-labeled neurophysin bound to polymethylmethacrylic particles was similarly injected into the animals. The half-time for the loss of radioactivity from samples of whole blood was 6.1–6.4 min as determined over the first 5 min after administration of the protein. There was no significant difference in the half-time calculated when the radioactivity present in the trichloroacetic acid-insoluble material present in the serum was measured. 15 min after the injection of labeled protein there was a maximum and massive uptake of radioactivity in the kidney consistent with this tissue’s being important in the degradation of neurophysin. Immunoperoxidase histochemical techniques were applied to formalin-fixed kidney slices and demonstrated the presence of neurophysin-like material localized in the cells of proximal tubules of the cortex and medulla. On binding neurophysin to acrylic particles there was approximately a 10-fold increase in the uptake of radioactivity in the lungs and a 33% reduction in activity in the kidneys, as measured at the 15-min time interval. Of the other tissues studied, excluding the thyroid gland and lungs, the uterus demonstrated the greatest uptake of radioactivity and fat tissue had the least accumulation of radioactive la
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