Histological Demonstration of Capillaries, Interstitial Space and Muscle Fibers in Heart and Skeletal Muscle with Fluorescent Dyes
作者:
F. Vetterlein,
G. Schmidt,
期刊:
Cells Tissues Organs
(Karger Available online 1983)
卷期:
Volume 116,
issue 4
页码: 285-289
ISSN:1422-6405
年代: 1983
DOI:10.1159/000145753
出版商: S. Karger AG
关键词: Myocardial capillaries;Myocardiβl fibers;Extracellular space;Skeletal muscle capillaries;Muscle fibers
数据来源: Karger
摘要:
A method is described which allows a clear demonstration of capillaries and muscle fibers in the heart and skeletal muscle of experimental animals. The fluorescent dyes fluorescein isothiocyanate (FITC) and lissamine rhodamine B 200 (RB 200) were conjugated with a protein of high (γ-globulin) and low (myoglobin) molecular weight, respectively, and were intravitally injected into the vascular system of rats. FITC globulin distributes itself in the intravasal space and RB 200 myoglobin in the extracellular. In histological sections the capillary lumina and the borderlines of the muscular fibers can be clearly identified and quantitatively evaluated because of the selective fluorescence in the respective structures
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