Salivary sIgA Response in HIV-1 Infection
作者:
Pia Skott,
Erik Lucht,
Inger Julander,
Joakim Dillner,
Ewa Björling,
期刊:
JAIDS Journal of Acquired Immune Deficiency Syndromes
(OVID Available online 1999)
卷期:
Volume 21,
issue 2
页码: 73-80
ISSN:1525-4135
年代: 1999
出版商: OVID
关键词: HIV-1;IgA;Envelope proteins;Mucosal immunity;Epitopes
数据来源: OVID
摘要:
Local and systemic production of total and HIV-1 specific IgA was determined in whole saliva and serum from 45 HIV-1-infected individuals and 15 healthy controls. The antigenic domains important for sIgA and IgG binding, respectively, was investigated with epitope mapping using synthetic peptides of HIV-1 proteins. Decreased levels of total sIgA in saliva were found among patients with low CD4+cell counts in advanced stages of acquired immunodeficiency. HIV-1 specific IgA response, predominantly directed to the envelope proteins, was found in saliva and serum also at later stages of disease. Analyses using peptide enzyme-linked immunosorbent assays (ELISA) showed that the sIgA antibody response in saliva was mainly directed to the V4 region (aa 385-409) and a more C-terminal part of the V3-region (aa 325-344) compared with the IgG response, which predominantly was directed to a more central part of the V3 loop (aa 308-325). A similar picture was seen for immunoglobulins of the two isotypes derived from serum. We have in this study shown IgA epitope-specific immune response within HIV-1 gp160, indicating that antibodies of IgA isotype may recognize somewhat different antigenic domains compared to IgG antibodies.
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