Summary:A noninstrumented enzyme immunochromatography (EIC) method for monitoring carbamazepine using whole blood was compared to the enzymemultiplied immunoassay technique (EMIT), fluorescence polarization immunoassay (FPIA), and high‐performance liquid chromatography (HPLC). Samples from 74 patients were evaluated in the comparison study, yielding correlation coefficients of 0.961 (EMIT), 0.974 (FPIA), and 0.867 (HPLC). The EIC method produced within‐run coefficients of variation of 4.3%, 4.9%, and 5.8% for three carbamazepine concentrations. The between‐run coefficient of variation over 107 days was 4.9%. The spiked serum sample analysis yielded recovery rates ranging from 98 to 102%. Enzyme immunochromatography was found to be a useful noninstrumented method for on‐site testing. The test gives quantitative patient sample results comparable to the results obtained using established laboratory methods.