Hydrolysis of Inorganic Pyrophosphate inDictyostelium discoideum
作者:
Kerstin Gezelius,
期刊:
Physiologia Plantarum
(WILEY Available online 1968)
卷期:
Volume 21,
issue 1
页码: 35-44
ISSN:0031-9317
年代: 1968
DOI:10.1111/j.1399-3054.1968.tb07228.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
AbstractThe hydrolysis of inorganic pyrophosphate has been studied in cell‐free extracts prepared at different stages of development ofDictyostelium discoideum. Two enzyme reactions, pH optima 7.25 and 9.0, appear specific for inorganic pyrophosphate and have an absolute requirement for a divalent cation, preferably Mg2+. The enzyme specific activities do not change significantly during differentiation. Neither enzyme reaction is inhibited by orthophosphate and the presence of exogenous potassium phosphate does not affect the levels of pyrophosphalase at any stage. Exogenous glucose raises the pyrophosphatase levels in the sorocarp
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