Localization of 5‐HT3receptors in the rat spinal cordimmunohistochemistry and in situ hybridization
作者:
Hossein Kia,
Marie-Christine Miquel,
Ruth McKernan,
Anne-Marie Laporte,
Marie-Christine Lombard,
Sylvie Bourgoin,
Michel Hamon,
Daniel Vergé,
期刊:
NeuroReport
(OVID Available online 1995)
卷期:
Volume 6,
issue 2
页码: 257-261
ISSN:0959-4965
年代: 1995
出版商: OVID
关键词: 5-HT, receptors;Immunohistochemistry;In situ hybridization;Spinal cord;Rat
数据来源: OVID
摘要:
SPECIFIC antibodies raised against a fusion protein containing the amino acid sequence of the putative second intracellular loop of the cloned 5-HT,-A receptor subunit were used for the immunohistochemical visualization of 5-HT, receptors in the rat spinal cord. A dense 5-HT,-like immunoreactivity was found in the superficial layers of the dorsal horn, which closely matched the labelling of 5-HT, binding sites by [125I]iodo-zacopride. This immuno-staining was markedly decreased following unilateral rhizotomy, consistently with a preferential location of 5-HT, receptors on terminals of primary afferent fibres, and with the presence of 5-HT, mRNA in dorsal root ganglia. However, a significant proportion of 5-HT, receptors persisted after rhizotomy, and the corresponding mRNA was found in the dorsal horn of the spinal cord. 5-HT, receptors are therefore also located on intrinsic neurones of the spinal cord.
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