Carbon transport was studied in vitro in preparations of trachea and bronchus from veal calves. The mean velocity varied from 4.2 mm/min in the main bronchi to 6.3 mm/min in the ventral trachea. In some locations no transport was observed. Stereomicroscopic evaluation of Alcian blue‐phloxine stained mucosal surfaces revealed differences in the appearance of the mucus layer between locations with and without mucus transport. It is concluded that quality and integrity of the mucus layer play an important role in mucus transport.