Comparison of the Neutral Red and Methylene Blue Assays to Study Cell Growth in Culture
作者:
ElliottW. Mark,
AuerspergNelly,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1993)
卷期:
Volume 68,
issue 1
页码: 29-35
ISSN:1052-0295
年代: 1993
DOI:10.3109/10520299309105573
出版商: Taylor&Francis
关键词: methylene blue;neutral red;cell growth
数据来源: Taylor
摘要:
The neutral red and methylene blue in vitro cytotoxicity assays were compared under a variety of conditions using normal human ovarian epithelial cells to determine whether either assay is superior for studying cell growth. The results were standardized against a DNA spectrofluorometric assay. Although the assays were equivalent in reflecting cell number, each has specific advantages: while neutral red discriminates between viable and dead cells, the methylene blue assay is more sensitive and easier to perform.
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