Influence of the temperature of a drift tube and flow rate of nitrogen as nebulizing gas with 100% hexane and 40% hexane plus 60% (5% H2O in 2-propanol) as mobile phases on the respones of ELS detector for selected standards of ethoxylated alcohols was examined. For optimal conditions (1.5 dm3N2/min, 50°C) calibration curves for both mobile phases were created. The response of ELSD for standards not undergoing evaporation is mainly affected by the composition of the mobile phase.