In VivoLability of Red Cell Phosphofructokinase in Term InfantsThe Possible Molecular Basis of the Relative Phosphofructokinase Deficiency in Neonatal Red Cells
作者:
SUSAN TRAVIS,
JAMES GARVIN,
期刊:
Pediatric Research
(OVID Available online 1977)
卷期:
Volume 11,
issue 11
页码: 1159-1161
ISSN:0031-3998
年代: 1977
出版商: OVID
关键词: Discontinuous density gradient;isozyme;phosphofructokinase;pyruvate kinase
数据来源: OVID
摘要:
SummaryCord blood erythrocytes from nine term infants were separated by density gradient centrifugation into cohorts of intact cells of progressively increasing density and compared with red cells treated in a similar manner from four healthy adults. Pyruvate kinase (PK), an age-dependent enzyme, progressively decreased in activity from the lightest to the heaviest fractions, in both neonatal and adult red cells, indicating that red cells from newborn infants exhibit the same relationship between red cell age and density that had previously been demonstrated in red cells from adults. The rate of decline of red cell PK activity was essentially the same in neonates and adults, whereas phosphofructokinase (PFK) activity in cord erythrocytes decreased at a significantly faster rate when compared to adults. These data suggest that PFK has an accelerated rate ofin vivodecay in neonatal red cells and is an unstable enzyme in the newborn.SpeculationThe finding that PFK from cord blood has an accelerated rate ofin vivodecay suggests that the relative PFK deficiency that exists in neonatal red cells is secondary to normal synthesis of an unstable enzyme, and leads to the speculation that red cell PFK in newborn infants is an isozyme (a “fetal” PFK).
点击下载:
PDF
(523KB)
返 回