C-banding technique was applied in some maize materials with the purpose of verifing cytogenetical association between chromosome 2S knob and the presence and expression oflatent-1supergene and also mitotic chromosome characterization. Materials chosen were IP 48-5-3 Cateto Assis Brasil, its isoline IP 48-5-3-lte-1carringlatent-1factor and Michoacan 21-1-104latent-1donnor. C-band number obtained was 3.26 ± 0.69 for IP 48-5-3 Assis Brasil, 7.86±1.57 for IP-48-5-3-lte-1and 8.43 ±1.12 for Michoacan 21-1-104. The mean of total heterochromatin amount values obtained and expressed as percentage were 4.07±0.46; 9.68±1.59 and 13.99±2.05, respectively, C-band was used because of good correspondence between heterochromatic knobs and positive C-bands. IP 48-5-3lte-1isoline and Michoacan 21 showed positive C-band on short arm of chromosome 2 and IP 48-5-3 Assis Brasil did not show any band in the same chromosome.