High Performance Liquid Chromatographic Separation of the Globin Chains of Non-Human Hemoglobins
作者:
SchroederW. A.,
SheltonJoan B.,
SheltonJ. Roger,
HuynhVan,
TeplowDavid B.,
期刊:
Hemoglobin
(Taylor Available online 1985)
卷期:
Volume 9,
issue 5
页码: 461-482
ISSN:0363-0269
年代: 1985
DOI:10.3109/03630268508997024
出版商: Taylor&Francis
数据来源: Taylor
摘要:
High performance liquid chromatography has been applied to the separation of the globin chains of 16 non-human species which include common mammalian and avian species. The procedure uses a large-pore C. column which has been effectively employed for the separation of human globin chains. in many cases, the gradient for human chains with trifluoroacetic acid-water-acetonitrile was satisfactory or required only moderate modification. The separations were excellent for all except dog hemoglobin. Many results substantiate prior published information about heterogeneity, amino acid composition, etc. of the individual hemoglobins. Additional data on some of these hemoglobins have also been obtained, and some previously unstudied hemoglobins have been examined.
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