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Microquantification of Proteins by Spectrophotometry. Part I: From 190 nm to 1100 nm, Selection of Wavelengths

 

作者: Pascal Baret,   Ariane Angeloff,   Claude Rouch,   Michel Pabion,   Frédéric Cadet,  

 

期刊: Spectroscopy Letters  （Taylor Available online 1997）
卷期: Volume 30, issue 6  

页码: 1067-1088

 

ISSN:0038-7010

 

年代: 1997

 

DOI:10.1080/00387019708006708

 

出版商: Taylor & Francis Group

 

关键词: far U.V.;protein microquantification;enzyme.

 

数据来源: Taylor

 

摘要:

Several methods for protein determination have been described. In almost all cases, a detection reagent is involved (Coomassie Blue reagent, bicinchoninic acid, Folin reagent). Proteins quantification determination by measurements in the U.V. region1have been abandonned because of the weak sensitivity of the apparatus (concentrations of the order of 100 μg/ml) and because of the lack of precision at low wavelengths (far U.V.).

 

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