Norepinephrine: Adenosinetriphosphate ratios in purified adrenergic vesicles
作者:
S.‐S. Yen,
R. L. Klein,
S.‐H. Chen‐Yen,,
Å. Thureson‐Klein,
期刊:
Journal of Neurobiology
(WILEY Available online 1976)
卷期:
Volume 7,
issue 1
页码: 11-22
ISSN:0022-3034
年代: 1976
DOI:10.1002/neu.480070103
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractNorepinephrine (NE):adenosinetriphosphate (ATP) ratios were studied in a highly purified fraction of large dense core vesicles isolated from the bovine splenic nerve. Vesicles prepared from nerves chilled ∼10 and 30 min post mortem were compared. The NE:ATP molar ratio decreased from 6.3 to 4.8,p<0.005; NE decreased from 61 to 42 nmol, while ATP decreased only from 9.6 to 8.8 nmol/mg protein. Animals weighing 180‐360 kg were compared with heavier ones weighing 400‐700 kg. NE increased from 42 to 68 nmol and ATP increased from 5.9 to 13.2 nmol/mg protein, while the NE:ATP molar ratio decreased from 7.2 to 5.2,p<0.005. Changes during vesicle maturation were studied by comparing vesicles identically prepared from equal weights of a proximal nerve segment close to the coeliac ganglion and a distal, intrasplenic segment. NE increased from 45 to 70 nmol while ATP remained unchanged at 10.0 nmol/mg protein and the NE:ATP molar ratio increased from 4.5 to 7.0,p<0.005. It was interpreted that vesicle ATP content, like dopamine β‐hydroxylase, was established early in the cell body and remained unchanged during axoplasmic transport. ATP was in a complex which was relatively stable to post mortem hydrolysis at least between 10 and 30 min prior to chilling the nerves. The addition of newly synthesized NE into a readily releasable pool during axoplasmic transport occurs without ATP and can account for the increased ratio above 4:1 in the distal segment
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