Modification of the Acid Elution Technique for Quantitation of Fetal Hemoglobin in Individual Erythrocytes
作者:
BernsteinS. C.,
BowmanJ. E.,
SwiftH. H.,
期刊:
Hemoglobin
(Taylor Available online 1977)
卷期:
Volume 1,
issue 4
页码: 313-331
ISSN:0363-0269
年代: 1977
DOI:10.3109/03630267708996893
出版商: Taylor&Francis
数据来源: Taylor
摘要:
A modification of the acid elution procedure for the demonstration of fetal hemoglobin in red cells using fast green stain is described. This technique not only permits improved visual estimation of the amount of fetal hemoglobin in red cells, but allows for the direct quantitation of fetal hemoglobin content of individual cells, using a scanning and integrating micro-densitometer. As an application of this method, the distribution of fetal hemoglobin in populations of red cells of individuals was determined. Distributions were examined in sickle cell anemia patients with different proportions of fetal hemoglobin.
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