Measurement of Plasma Total Renin by the Anti-Human Renin Monoclonal Antibodies
作者:
KOH-ICHI SUGIMOTO,
HIROSHI SHIONOIRI,
KOHSUKE MINAMISAWA,
YASUHIKO ABE,
SHIN-ICHIRO UEDA,
EIJI GOTOH,
MASAO ISHII,
HIDEO HIMENO,
TOSHIYOSHI TOSHIYOSHI,
期刊:
The American Journal of the Medical Sciences
(OVID Available online 1991)
卷期:
Volume 302,
issue 6
页码: 342-346
ISSN:0002-9629
年代: 1991
出版商: OVID
关键词: Plasma total renin;Plasma active renin;Direct RIA;Essential hypertension;Renovascular hypertension;Primary Aldosteronism;Diabetes mellitus
数据来源: OVID
摘要:
The authors evaluated the assay performances and clinical usefulness of a newly developed solid phase radioimmunoassay (RIA) for total renin concentration (TRC) in human plasma. The direct total renin RIA was performed by a sandwich technique with a pair of anti-human renin monoclonal antibodies. Renin activation with trypsin did not change TRC. The RIA showed satisfactory assay performances and demonstrated full compatibility with a direct RIA-kit for active renin concentration (ARC) in human plasma. The values of TRC were 105.3 ± 8.6 pg/mL in normal subjects and 136.5 ± 14.6 pg/mL in patients with essential hypertension. The values of TRC and the ratios of ARC to TRC were high in patients with renovascular hypertension and were low in patients with primary aldosteronism. Although the TRC value in diabetic patients was 134.4 ± 14.8 pg/mL, the ratio of ARC to TRC was low. The RIA procedure was simple since prior purification or activation of renin was not required. These results suggest that the total renin RIA and its combined use with the active renin RIA may be helpful in understanding the renin-angiotensin system in human plasma.
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