Differential Silver Enhanced Double Labeling in Immunoelectron Microscopy
作者:
SlaterMichael,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1991)
卷期:
Volume 66,
issue 3
页码: 153-154
ISSN:1052-0295
年代: 1991
DOI:10.3109/10520299109110570
出版商: Taylor&Francis
关键词: immunoelectron microscopy;silver enhancement;pituitary
数据来源: Taylor
摘要:
A two-sided double labeling method using protein A gold was used to demonstrate the presence of two hormones within the same anterior pituitary cell granule. A single probe size was used for both section faces but one side of the grid was silver enhanced. The use of a single probe size reduced the cost of the study and eliminated the variations in labeling efficiency that result from the use of different probe sizes.
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