首页   按字顺浏览 期刊浏览 卷期浏览 Silver Enhancement of Colloidal Gold Particles in Deplasticized Semithin Epoxy Sections
Silver Enhancement of Colloidal Gold Particles in Deplasticized Semithin Epoxy Sections

 

作者: GualtieriR.,   AndreuccettiP.,  

 

期刊: Biotechnic&Histochemistry  （Taylor Available online 1995）
卷期: Volume 70, issue 1  

页码: 7-11

 

ISSN:1052-0295

 

年代: 1995

 

DOI:10.3109/10520299509108309

 

出版商: Taylor&Francis

 

关键词: gold labeling;pre-embedding;silver enhancement;deplasticized sections;biotinylated lectins;streptavidin-gold;amphibian egg

 

数据来源: Taylor

 

摘要:

A simple method for light microscope screening of pre-embedded, gold-labeled samples, by silver enhancing deplasticized semithin epoxy sections is presented. This method is of great help in visualizing the topography of gold labeled sites at low magnification and for selecting labeled sample regions for subsequent electron microscope analysis on serial ultrathin sections. The method was developed during a study of lectin binding sites on the egg surface of the anuran amphibianDiscoglossus pictus

 

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