Flow-Cell Fibre-Optic Enzyme Sensor for Phenols
作者:
DmitryB. Papkovsky,
AndreyL. Ghindilis,
IlyaN. Kurochkin,
期刊:
Analytical Letters
(Taylor Available online 1993)
卷期:
Volume 26,
issue 7
页码: 1505-1518
ISSN:0003-2719
年代: 1993
DOI:10.1080/00032719308017428
出版商: Taylor & Francis Group
关键词: Laccase;Polyphenols;Chlorophenols;Sensor;Fibre-optic;Phosphorescence;Oxygen;FIA
数据来源: Taylor
摘要:
A solid-state fibre-optic luminescent oxygen sensor was used for flow-through measurements. It acts as a transducer in a new flow-cell enzyme sensor arrangement. This arrangement comprises a flow path, sample injector, microcolumn with the immobilized enzyme, oxygen membrane and fibre-optic connector joined together to form an integral unit. Laccase enzyme was used as a recognition system which provided specific oxidation of the substrates with the dissolved oxygen being monitored. The assay procedure was optimized and performance of the new system studied. The sensor was applied to the determination polyphenol content in tea, brandy, etc. (quality control test). The sensitivity to some important phenolic compounds was tested with the view of industrial wastewater control applications.
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