Phosphate solubilizing ability of different isolates of fungi associated with legume root nodules was studied in vitro. Among the fungi tested, isolates ofPenicillium lilacinum,Aspergillussp.,A.flavus,A.niger,A.terreus, andA.nidulanssolubilized insoluble tricalcium phosphate. When soluble potassium dihydrogen phosphate was present with tricalcium phosphate in the medium, some of the fungi failed to solubilize phosphate.