DEVELOPMENT OF AN HPLC/DIODE ARRAY DETECTOR METHOD FOR THE DETERMINATION OF HUMAN PLASMA ADENOSINE CONCENTRATIONS
作者:
R. Guieu,
F. Sampieri,
G. Bechis,
G. Halimi,
B. Dussol,
Y. Berland,
J. Sampol,
H. Rochat,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1999)
卷期:
Volume 22,
issue 12
页码: 1829-1841
ISSN:1082-6076
年代: 1999
DOI:10.1081/JLC-100101769
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Assays of adenosine and its derivatives in biological fluids involve fluorimetric, radioimmunological or chromatographic analyses. Techniques currently used are tedious because they involve either an extraction using immunological methods or one or two freeze drying cycles when using high performance liquid chromatography (HPLC). In this context, we describe a “quick” HPLC method using a diode array detector for the spectral analysis and quantitation of adenosine and its derivatives in less than two hours following sampling. We compared our results to those obtained with an HPLC technique coupled to UV detection.
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