Incorporation of14C acetate into the sterols of rabbit lens in organ culture
作者:
AlbersBarbara,
FarrisBarbara D.,
SwindellRobert T.,
期刊:
Current Eye Research
(Taylor Available online 1982)
卷期:
Volume 2,
issue 9
页码: 607-612
ISSN:0271-3683
年代: 1982
DOI:10.3109/02713688208996361
出版商: Taylor&Francis
数据来源: Taylor
摘要:
Rabbit lenses were incubated in organ culture with14C acetate for 3, 6, 12 and 20 hours and the nonsaponifiable fraction, composed primarily of sterols, was isolated. The incorporation of acetate into the nonnaponifiable fraction was linear for periods of 3 to 20 hours incubation. The nonsaponifiable fraction was subjected to thin-layer chromatography and 6 bands were observed. On the basis of Rfvalues, one band was tentatively identified as squa-lene, another as lanosterol and a third as a coholesterol-desmosterol mixture. The percentage of the radioactivity incorporated into the eholesterol-desmosterol band increased with incubation time. Conversely the percentage of the radioactivity incorporated into the squalene and lanosterol bands decreased with incubation time, indicating a precursor-product relationship.
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