Nothoscordum fragranschromosomes were mildly prepared by enzyme maceration and subsequent air-drying. Cytological characteristics of heterochromatic segments were studied by the C-banding technique, fluorochrome-staining and treatment with chemicals. Results showed that at least two classes of heterochromatic segments were differentiated with regard to the base composition of DNA and treatment with various chemicals or enzyme solubilized the heterochromatic segments comprised of GC-rich DNA.