Simultaneous Determination of Carbamazepine, Phenobarbital and Their Major Metabolites in Serum, Brain Tissue and Urine by High-Performane Liquid Chromatographya
作者:
Estetama Mendez-alvarez,
Ramon Soto-otera,
German Sierra-marcuňo,
期刊:
Analytical Letters
(Taylor Available online 1987)
卷期:
Volume 20,
issue 8
页码: 1275-1292
ISSN:0003-2719
年代: 1987
DOI:10.1080/00032718708064608
出版商: Taylor & Francis Group
关键词: Carbamazepine;phenobarbital;high-performance liquid chromatography;serum;brain tissue;urine
数据来源: Taylor
摘要:
A high-performance liquid chromatographic method is described for the simultaneous measurement of carbamazepine, phenobarbital and their major metabolites in small samples of serum, brain tissue and urine. This involves solvent extraction, reversed-phase chromatography and ultraviolet detection at 195 nm. Glucuronides in urine are hydrolyzed by enzymatic cleavage with β-glucuranidase. Quantitation is based on the peak-height ratio of the analyte to its internal standard (10, 11-dihydrocarbamaze-pine or p-methylphenobarbital). The results obtained show that the method is precise and reproducible.
点击下载:
PDF (489KB)
返 回