Hexamethyldisilazane as a Drying Agent for Pollen Scanning Electron Microscopy
作者:
ChissoeWilliam F.,
VezeyEdward L.,
SkvarlaJohn J.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1994)
卷期:
Volume 69,
issue 4
页码: 192-198
ISSN:1052-0295
年代: 1994
DOI:10.3109/10520299409106286
出版商: Taylor&Francis
关键词: hexamethyldisilazane;HMDS;Pollen;SEM
数据来源: Taylor
摘要:
Use of hexamethyldisilazane (HMDS) as a final dehydrating solution provides robust, undistorted secondary electron images of a variety of angiosperm and gymnosperm pollen grains, including those considered to be susceptible to collapse in the scanning electron microscope. Ease of handling, low cost, lack of specialized equipment, minimal expenditure of time, and high rate of success are factors that favor HMDS over other drying agents for preparing pollen grains for scanning' electron microscopy.
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