Genetic Effects on the Longevity of Cultured Human Fibroblasts. III
作者:
R. Holliday,
K.V.A. Thompson,
期刊:
Gerontology
(Karger Available online 1983)
卷期:
Volume 29,
issue 2
页码: 89-96
ISSN:0304-324X
年代: 1983
DOI:10.1159/000213098
出版商: S. Karger AG
关键词: Glucose-6-phosphate dehydrogenase;Fibroblasts;Ataxia telangiectasia;Fanconi’s anaemia;Werner’s syndrome;Bloom’s syndrome;Progeria;Cockayne’s syndrome;Paromomycin
数据来源: Karger
摘要:
The level of heat-labile glucose-6-phosphate dehydrogenase (G6PD) has been measured in skin fibroblast cultures from premature ageing or DNA repair deficient genetic syndromes. The short in vitro longevity of Werner’s syndrome, progeria, Cockayne’s syndrome, ataxia telangiectasia, Fanconi’s anaemia, and Bloom’s syndrome cultures was correlated with the appearance of a significant fraction of heat-labile enzyme. Long-lived control cultures contain a low level of altered enzyme until they become senescent. The evidence that heat-labile G6PD molecules are derived from errors in synthesis, or from other causes, is critically assessed. It is shown that normal cells grown in medium containing the antibiotic, paromomycin, which is known to reduce the fidelity of ribosomal translation, produce a significant fraction of alter
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