A chilling‐sensitive mutant ofArabidopsisis deficient in chloroplast protein accumulation at low temperature*
作者:
J. C. SCHNEIDER,
E. NIELSEN,
C. SOMERVILLE,
期刊:
Plant, Cell&Environment
(WILEY Available online 1995)
卷期:
Volume 18,
issue 1
页码: 23-32
ISSN:0140-7791
年代: 1995
DOI:10.1111/j.1365-3040.1995.tb00540.x
出版商: Blackwell Publishing Ltd
关键词: Arabidopsis thaliana;chilling sensitivity;chloroplast protein
数据来源: WILEY
摘要:
ABSTRACTMutations at thechsllocus inArabidopsis thaliana(L.) confer a chilling‐sensitive phenotype in which plants become chlorotic and die after more than 3 d of exposure to temperatures in the range of 18 to 10°C. Within 8h after transfer of mutant plants from growth at 23 to 13°C, accumulation of the newly synthesized chloroplast‐localized polypeptides stearoyl‐ACP desaturase, glutamine synthetase and OEC 23 was severely impaired. By contrast, there was no apparent deleterious effect on the accumulation of two extrachloroplastic proteins examined: the elongation factor lα, and the phloem‐specific, extrachloroplastic isoform of glutamine synthetase. In one instance examined in detail, chilling did not decrease the accumulation of mRNA for stearoyl‐ACP desaturase, indicating that the effect was post‐transcriptional. Chloroplasts isolated from chilled wild‐type andchslplants were equally able to process and protect from protease digestionin vitrosynthesized pre‐plastocyanin. The results suggest that thechslmutant may be defective in some aspect of chloroplast protein accumulation
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