Transmission of Three Viroids Through Seed and Pollen of Tomato Plants
作者:
S. Kryczyński,
E. Paduch‐Cichal,
L. J. Skrzeczkowski,
期刊:
Journal of Phytopathology
(WILEY Available online 1988)
卷期:
Volume 121,
issue 1
页码: 51-57
ISSN:0931-1785
年代: 1988
DOI:10.1111/j.1439-0434.1988.tb00952.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
AbstractThe severe strain of potato spindle tuber viroid (s‐PSTV) as well as chrysanthemum stunt (CSV) and cucumber pale fruit (CPFV) viroids were found to be transmitted through seed and pollen of the tomato cvs. Rutgers and Najwcześniejszy. Plants pollinated with a pollen infected with any of these three viroids became systematically infected. Plant, fruit and seed symptoms of viroid infection were noted on sap‐ and pollen‐inoculated plants and the yield of these plants was reduced.Tomato cv. Rutgers plants grown from infected seeds were symptomless although all three viroids were detected in these plants by bioassay and by electrophoresis on 5% polyacrylamide gel.When DNA complementary to s‐PSTV RNA was used for a direct viroid detection in seed samples by spot hybridization technique it hybridized not only with s‐PSTV RNA but also with CSV RNA as well as wit
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