Differentiation and Activation Associated Expression of IL-6 and IL-6 Receptors in U-937 Monocytic Cells: Relationship to the Expression of CD14
作者:
ÖbergFredrik,
NilssonKenneth,
期刊:
Growth Factors
(Taylor Available online 1992)
卷期:
Volume 7,
issue 1
页码: 85-96
ISSN:0897-7194
年代: 1992
DOI:10.3109/08977199209023940
出版商: Taylor&Francis
关键词: U-937;differentiation;IL-6;IL-6R;LPS;CD14
数据来源: Taylor
摘要:
AbstractActivated monocytes play an important role as producers of IL-6 during inflammation and immune response. We show that during monocytic differentiation of U-937 cells, induced by phorbolester (PMA), IL-6 mRNA expression was transiently up-regulated and IL-6 protein was secreted into the medium. In contrast, differentiation induced by VitD3 or Retinoic acid (RA) did not lead to an increase in the IL-6 expression. Thus, IL-6 expression does not seem to be associated with monocyte differentiationper se. However, U-937 cells terminally differentiated by VitD3, rapidly responded to bacterial lipopolysaccharide (LPS) induced activation by IL-6 expression and secretion. In cells, differentiated by PMA, the IL-6 expression was super-induced after activation by interferon-γ(IFN-γ) and LPS. The capacity of U-937 cells to respond to LPS activation by IL-6 expression was associated with the expression of CD14 and some serum components(s) were a prerequisite for a successful LPS induction. The IL-6R expression was down-regulated during monocytic differentiation of U-937 cells. In the terminally differentiated U-937 cells, the expression of IL-6R could be induced after activation by IFN-γand to a lesser extent by LPS, suggesting a mechanism by which activation positively regulates the response to IL-6 in macrophages.
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