Reciprocal Cellular Distribution of Glucagon‐like Peptide‐1 (GLP‐1) Immunoreactivity and GLP‐1 Receptor mRNA in Pancreatic Islets of Rat
作者:
Dieter Hörsch,
Rüdiger Göke,
Rolf Eissele,
Bettina Michel,
Burkhard Göke,
期刊:
Pancreas
(OVID Available online 1997)
卷期:
Volume 14,
issue 3
页码: 290-294
ISSN:0885-3177
年代: 1997
出版商: OVID
关键词: Pancreas;Receptor physiology;Glucagon-like peptide-1;α cells;β cells
数据来源: OVID
摘要:
The respective cellular distribution of glucagon-like peptide-1 (GLP-1) immunoreactivity and mRNA expression of the GLP-1 receptor was compared in rat pancreas by means of immunohistochemistry and in situ hybridization. GLP-1 immunoreactivity was present in the marginal zone of rat pancreatic islets. In contrast, GLP-1 receptor mRNA signals were confined to the central part of pancreatic islets. Neither GLP-1 immunoreactivity nor GLP-1 receptor mRNA signals were detected in the exocrine pancreatic acinar cells or duct cells. The differential distribution of GLP-1 immunoreactivity and GLP-1 receptor mRNA signals indicates that the GLP-1 amino acid sequence is present in the α-cell zone of pancreatic islets, whereas the GLP-1 receptor is expressed mainly by α cells. Thus, our data by in situ hybridization demonstrates the significant expression of GLP-1 receptors on β cells but makes a significant expression on α cells rather unlikely.
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