Rosemary oil was produced from plants grown in two different zones of Argentina (Sumalao and Castelar). The highest yield of fresh plant material and oil was found in Sumalao. The physico-chemical properties, chemical composition and odor quality of the Argentinian oils were very similar to commercial oil, in particular Portuguese oils. Twenty compounds were identified in the oils, of which the major ones were α-pinene (7.9–10.9%), myrcene (17.9–20.4%), 1,8-cineole (14.5–15.3%) camphor (9.0–9.3%) and β-caryophyllene (14.5–15.3%). Some differences were observed in chemical composition of two oils produced by different methods of distillation.