Protease production by immobilized mycelia ofStreptomyces fradiae
作者:
Toshinori Kokubu,
Isao Karube,
Shuichi Suzuki,
期刊:
Biotechnology and Bioengineering
(WILEY Available online 1981)
卷期:
Volume 23,
issue 1
页码: 29-39
ISSN:0006-3592
年代: 1981
DOI:10.1002/bit.260230104
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractStreptomyces fradiaewas immobilized in polyacrylamide gel prepared from 5% total acrylamide (90% acrylamide and 10%N,N′‐methylenebisacrylamide). Production of protease by the immobilized mycelia was attempted in a batch system. A dilute medium containing 0.5% starch, 0.5% meat extract, and 0.05% yeast extract was employed. The reusability of the immobilized and washed mycelia was examined. The activity of protease production by washed mycelia was rapidly decreased with increasing use cycles. The activity of the immobilized mycelia increased gradually, and reached a maximum after ten use cycles. Then, the activity gradually decreased with increasing reaction cycles. This might be caused by destruction of the gels. On the other hand, the sterilization of the surface of the immobilized mycelia was effective for elongation of the lifetime. As a result, the half‐life of protease production by the sterilized immobilized mycelia was about 30 days. The rate of protease production by immobilized mycelia was 12,000 U/ml/hr. This value was four times higher than that by submerged cu
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