Mechanism of Immobilization of Enzymes by Radiation-Induced Polymerization of Glass-Forming Monomers
作者:
Masaru Yoshida,
Minoru Kumakura,
Isao Kaetsu,
期刊:
Journal of Macromolecular Science: Part A - Chemistry
(Taylor Available online 1980)
卷期:
Volume 14,
issue 4
页码: 541-554
ISSN:0022-233X
年代: 1980
DOI:10.1080/00222338008081034
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Immobilization of enzymes by radiation-induced polymerization has been studied at low temperatures by use of various hydrophilic and hydrophobic glass-forming monomers such as hydroxy-alkyl methacrylate and poly(ethylene glycol diacrylate). Activity yield of the immobilized enzyme depends on the monomer concentration in polymerization. In the immobilized enzymes with strongly hydrophobic matrices, the activity shows a maximum at an optimum monomer concentrations in a certain stage of repeated usage for the enzyme reaction. The decrease of activity with repeated use is very little in strongly hydrophobic systems, particularly in diethylene glycol diacrylate polymer matrices. The hydrophobic polymer-enzyme composite has the microsphere form. In the present method, a model scheme for immobilization mechanism is proposed, which is compared with that formed in the polymerization of a nonglass-forming monomer system and also by the solution polymerization at higher temperatures.
点击下载:
PDF (717KB)
返 回