Changes in interferon‐regulatory factor‐1 mRNA levels after transient ischemia in rat brain
作者:
Wulf Paschen,
Cornelia Gissel,
Sonja Althausen,
Jens Doutheil,
期刊:
NeuroReport
(OVID Available online 1998)
卷期:
Volume 9,
issue 14
页码: 3147-3151
ISSN:0959-4965
年代: 1998
出版商: OVID
关键词: Calcium stores;Endoplasmic reticulum;Gene expression;Interferon;Irf-1;Mechanisms;Thapsigargin;Transient cerebral ischemia
数据来源: OVID
摘要:
TO evaluate whether the interferon system in the brain is activated by a severe form of metabolic stress, and to elucidate the possible mechanism underlying this activation, changes in the interferon regulatory factor-1 (irf-1) mRNA levels were evaluated after transient cerebral ischemia, and after exposure of primary neuronal cells to experimental conditions resulting in a depletion of ER calcium stores. Following 30 min ischemia and 2 h recovery, irf-1 mRNA levels rose significantly and stayed high for up to 24 h of recovery. Irf-1 mRNA levels were also significantly increased in neuronsin vitroafter exposing cells to conditions resulting in ER calcium store depletion with or without a parallel increase in cytoplasmic calcium activity. It is concluded that transient cerebral ischemia induces activation of the interferon system and that disturbances of ER calcium homeostasis may play a role in this process.
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