Rapid In Situ Cellular Kinetics of Intracerebral Tumor Angiogenesis Using a Monoclonal Antibody to Bromodeoxyuridine
作者:
Susan Brien,
David Zagzag,
Steven Brem,
期刊:
Neurosurgery
(OVID Available online 1989)
卷期:
Volume 25,
issue 5
页码: 715-719
ISSN:0148-396X
年代: 1989
出版商: OVID
关键词: Angiogenesis;Brain tumors;Bromodeoxyuridine;Cytokinetics;Endothelial cell;Immunohistochemistry;Monoclonal antibody
数据来源: OVID
摘要:
&NA;The application of a monoclonal antibody to bromodeoxyuridine (BUdR) provides a rapid, reproducible, nontoxic, immunohistochemical method to measure cellular kinetics of intracerebral tumor angiogenesis. The rabbit brain tumor model of angiogenesis consists of tumor and endothelial cell populations with high proliferative rates that demonstrate the close interdependence between microvascular and neoplastic growths as well as topographic gradients, heterogeneity, and regional microdomains of cell proliferation. The labeling index (LI) of endothelial cells was 25.8% at the tumor periphery, compared to 1.7% in the tumor center (P< 0.001). Concomitant with an increased turnover of neoplastic cells at the tumor periphery, LI was 26.6% with a LI of 7.7% in the center (P< 0.01). Furthermore, labeled tumor cells tended to be organized around proliferating capillaries, with less DNA synthesis farther from the nearest blood vessel. The established normal microvessels of the brain, e.g., in the opposite tumor‐free hemisphere, were mitotically inactive with a LI of <0.001%. Quantitation of vascular cytokinetics should be useful in further studies of the pathophysiology of brain tumor angiogenesis and the development of pharmacological approaches directed toward the microvasculature. (Neurosurgery25:715‐719, 1989)
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