Development of a Laser Nephelometric Method for the Quantitation of Human Glycohemoglobins
作者:
PeterP. Chou,
Julius Kerkay,
ManjulaK. Gupta,
期刊:
Analytical Letters
(Taylor Available online 1981)
卷期:
Volume 14,
issue 13
页码: 1071-1087
ISSN:0003-2719
年代: 1981
DOI:10.1080/00032718108081448
出版商: Taylor & Francis Group
关键词: Human Glycohemoglobins;Hemoglobin A1;Antiserum to Hemoglobin A1;Diabetes Mellitus;Ion-Exchange Liquid Chromatography;Laser Nephelometry
数据来源: Taylor
摘要:
A nephelometric procedure for quantitative measurement of glycohemoglobin (Hb A1) was developed, evaluated, and compared with the semi-quantitative mini-column chromatographic procedure. Hb A1was purified from human red cell hemolystate by Bio-Rex 70 ion-exchange liquid chromatography and was used for standards and immunization. The antisera raised in rabbits showed high cross-reactivities with normal human hemoglobin (Hb A). The latter was separated by mixing 25 μl of patients' hemolystate with 2 ml ion-exchange resin suspension for 15 minutes. One hundred microiters of the supernatant was incubated with 900 μl antiserum (dilution 1:50) for 45 minutes at room temperature. Samples were then read on a laser nephelometer. The sensitivity of the assay was found to be 0.1 mg Hb A1. The intraassay relative standard deviation (RSD) was 5.6% and the interassay RSD was 6.5%.
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