A number of milk samples collected in Egypt from different animal species and at different locations were analyzed for the presence of the organochloride pesticides aldrin and dieldrin. A simple competitive enzyme‐linked immunosorbent assay (ELISA) was used for the detection and quantification of aldrin and dieldrin in milk samples from different species: buffalo, cow, goat, sheep and donkey. Pesticides were detected in 62.5% (10/16) of the buffalo milk samples, 73.33% (11/15) of the cows’ milk samples, 25% (3/12) of the goats’ milk samples, 71.42% (5/7) of the sheeps’ milk samples and 66–66% (2/3) of the donkeys’ milk samples.