Mode of action of pediocin AcH fromPediococcus acidilacticiH on sensitive bacterial strains
作者:
A.K. Bhunia,
M.C. Johnson,
B. Ray,
N. Kalchayanand,
期刊:
Journal of Applied Bacteriology
(WILEY Available online 1991)
卷期:
Volume 70,
issue 1
页码: 25-33
ISSN:0021-8847
年代: 1991
DOI:10.1111/j.1365-2672.1991.tb03782.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
The peptide, pediocin AcH, fromPediococcus acidilacticiH binds to the cell surface ofLactobacillus plantarumNCDO 955, its resistant mutant and several other sensitive and resistant Gram‐positive bacteria but not to Gram‐negative bacteria. Sensitive cells, following treatment with pediocin AcH, lost intracellular K ions, u.v.‐absorbing materials, became more permeable to ONPG and, in some strains, lysed. Binding of pediocin AcH was maximum at pH 6.0. Anions of several salts inhibited binding of pediocin AcH but this was overcome by increased concentrations of pediocin AcH. Treatment of sensitive cells with 1% SDS, 4 mol/1 guanidine‐HCl, several organic solvents and enzymes did not reduce subsequent binding of pediocin AcH. Partially purified cell wall from a sensitive strain was also able to bind pediocin AcH. However, treatment of the cell walls to remove lipoteichoic acid prevented binding. These molecules might, therefore, be one of the binding sites of pedio
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