LIVER ISCHEMIA AND REPERFUSION INDUCES A SYSTEMIC INFLAMMATORY RESPONSE THROUGH KUPFFER CELL ACTIVATION
作者:
Guido,
Wanner Wolfgang,
Ertel Peter,
Müller Yvonne,
Höfer Rosmarie,
Leiderer Michael,
Menger Konrad,
期刊:
Shock
(OVID Available online 1996)
卷期:
Volume 5,
issue 1
页码: 34-40
ISSN:1073-2322
年代: 1996
出版商: OVID
数据来源: OVID
摘要:
ABSTRACT—To study the role of Kupffer cells (KC) as a cellular source of proinflammatory cytokines in hepatic ischemia/reperfusion, Sprague-Dawley rats were subjected to 20 min global hepatic ischemia. Sham-operated animals served as controls. Blood levels of tumor necrosis factor-α (TNF-α), interleukin-1α (IL-α), and interleukin 6 (IL-6) were determined after 10, 30, 60, 120, and 240 min of reperfusion and compared with spontaneous cytokine release by KC isolated after 60 min of reperfusion. Hepatic ischemia/ reperfusion resulted in an enhanced (p< .01) spontaneous release of TNF-α (+482%), IL-1α (+33%), and IL-6 (+175%) by KC. Kinetic analysis of cytokinemia revealed an early increase (p< .01) of TNF-α and IL-1α within minutes upon reperfusion, while an elevation of IL-6 serum levels was observed with a delay of 2 h. Early cytokinemia was associated with dysfunction/injury of the liver, lung, and kidney after 4 and 24 h of reperfusion, respectively. These data indicate that hepatic ischemia/reperfusion results in Kupffer cell activation and increased cytokine levels, which may produce systemic inflammation and may be responsible for tissue injury locally and on remote sites.
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