HIV‐1 infection and expression in human colonic cellsinfection and expression in CD4+ and CD4− cell lines
作者:
M. Omary,
David Brenner,
Louise de Grandpre,
Kenneth Roebuck,
Douglas Richman,
Martin Kagnoff,
期刊:
AIDS
(OVID Available online 1991)
卷期:
Volume 5,
issue 3
页码: 275-282
ISSN:0269-9370
年代: 1991
出版商: OVID
关键词: HIV-1;nuclear factor χB;tumor necrosis factor α;long terminal repeat;CD4;phorbol 12-myristate 13-acetate;human colon cells;SW620;HT29;T84
数据来源: OVID
摘要:
Three human colonic epithelial cell lines, SW620, HT29, and T84, were characterized with respect to HIV-1 infection and gene expression. SW620 and HT29, but not T84, could be infected with HIV-1. CD4 messenger RNA and its protein product were identified in SW620 cells but not in HT29 or T84 cells. Anti-CD4 antibody blocked infection of SW620 cells but had no effect on infection of HT29 cells. In SW620 and HT29 cells transfected with the HIV-1 long terminal repeat (LTR) linked to the chloramphenicol acetyl transferase (CAT) reporter gene, an intact HIV-1 enhancer element was required for stimulation of CAT activity by tumor necrosis factor α (TNFα) and phorbol ester. T84 was not able to mediate a TNFα or phorbol ester response. These studies provide further evidence that HIV-1 can infect cells by mechanisms other than those mediated by the CD4 receptor and describe complementary models for analyzing HIV-1 infection and expression in colonic epithelial cells.
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