We measured glucose metabolism in five dogs before and 3 weeks after cardiac dener-vation; after this time myocardial norepinephrine is depleted. The discharge by the myocardium, of14CO2from infused 14C-D-glucose (U), decreased following denervation (P= 0.05). The ratio of14CO to total CO2 production, which measured the proportion of glucose to total substrate oxidized, also decreased following denervation (P = 0.05). The inhibition of glucose oxidation by denervation was not due to an increase in arterial lactate concentration. There was an associated increase in myocardial content of fructose-6-phosphate in an additional seven dogs (P< 0.01). We postulate that myocardial tissue norepinephrine is one of the controllers of the activity of phosphofructokinase. Circ Res 47: 338-345, 1980