Labelling of Di-iodofluorescein with Iodine-131 by Ultraviolet Excitation
作者:
M. Raich,
E. M. Mikhail,
期刊:
Isotopenpraxis Isotopes in Environmental and Health Studies
(Taylor Available online 1986)
卷期:
Volume 22,
issue 8
页码: 276-279
ISSN:0021-1915
年代: 1986
DOI:10.1080/10256018608623669
出版商: Taylor & Francis Group
关键词: excitation;fluorescein;iodine compounds;iodine 131;labelled compounds;labelling;ultraviolet radiation
数据来源: Taylor
摘要:
The labelling of diiodofluorescein with131I using the ultraviolet waves as an excitation energy has been tried. Different normal alcohols have been used as solvents. The different parameters affecting the labelling yield such as, presence of water in different proportions, pH of the medium and presence of different concentrations of carrier, have been studied. Also, the effective lines of the Hg lamp have been elucidated. The labelling mechanism in different conditions has been explained. The study revealed that higher yields were obtained when using a mixture of ethanol and water as solvent at pH ∼ 5.
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