Abstract.Under controlled laboratory conditions of 28–30oC and 16:8 L:D photoperiod, an attempt was made to develop an age‐grading technique forAnopheles culicifaciesmales. Mating activity was maximal when females were 5–12 days old and males were 5–7 days old. The numbers of total and mature spermatocysts declined significantly with age, and the proportion of the testes occupied by the sperm reservoir increased as virgin males grew older. Mating resulted in the loss of spermatozoa and accessory gland substance from the reproductive system. Loss of mating ability of older virgin males seemed to be age‐related, because the reproductive system contained ample supplies of accessory gland substance and spermatozoa. Morphological changes of the reproductive system, due to mating and age, were used to infer the age and reproductive history of unknown males in a laboratory e